Human Tumor Necrosis Factor, TNF Primacu™ ELISA Kit

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Blood coagulation
Metabolism
Cancer
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Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
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Immunology

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Home > Product > ELISA Primacu > Human Tumor Necrosis Factor, TNF Primacu™ ELISA Kit

Human Tumor Necrosis Factor, TNF Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.46 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 4.46 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Tumor necrosis factor

Code

BPE379

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

4.46 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.TNF-alpha Protein & Antibody Cancer Immunotherapy Immune Checkpoint Immunotherapy Targeted Therapy

UniProt accession

P01375

MASS（Da）

25644

GeneID

7124

Synonyms

TNFA;TNFSF2

Gene names

TNF

Research area

Cancer,Cardiovascular,Immunology,Metabolism,Microbiology,Neuroscience,Signal transduction,Stem cells

Target protein

TNF-alpha

Components

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

Protocol data

These standard curves of BPE379 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1500 pg/ml	2.316	2.427	2.346
	2.538
750 pg/ml	1.249	1.25	1.169
	1.25
375 pg/ml	0.625	0.652	0.571
	0.678
187.5 pg/ml	0.37	0.364	0.283
	0.357
93.75 pg/ml	0.206	0.219	0.138
	0.231
46.875 pg/ml	0.135	0.143	0.062
	0.15
23.4375 pg/ml	0.109	0.107	0.026
	0.104
0 pg/ml	0.077	0.081	0
	0.084

Precision

We measured random samples of BPE379 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	264	6.8	2.6
Intra-assay	2	20	575	22.7	3.9
Intra-assay	3	20	1173	46	3.9
Inter-assay	1	20	264	21.5	8.2
Inter-assay	2	20	578	46.4	8
Inter-assay	3	20	1175	69.8	5.9

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

5*96T

BPE379

$2040.00
96T

BPE379

$480.00
15*96T

BPE379

$5400.00

Add to Cart

Lead time: Within two weeks

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