Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE143 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200 pg/ml	2.598	2.591	2.481
	2.584
600 pg/ml	1.438	1.372	1.262
	1.306
300 pg/ml	0.802	0.77	0.66
	0.738
150 pg/ml	0.437	0.447	0.337
	0.457
75 pg/ml	0.282	0.272	0.162
	0.262
37.5 pg/ml	0.189	0.191	0.081
	0.193
18.75 pg/ml	0.147	0.15	0.04
	0.153
0 pg/ml	0.112	0.11	0
	0.108

We measured random samples of BPE143 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	70.5	4.8	6.8
Intra-assay	2	20	160	5.6	3.5
Intra-assay	3	20	696	33.1	4.8
Inter-assay	1	20	70.7	4.3	6.1
Inter-assay	2	20	161	8.1	5
Inter-assay	3	20	704	39.8	5.7

> Protocol Booklet

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