Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE217 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200 pg/ml	2.89	2.987	2.868
	3.083
600 pg/ml	1.389	1.428	1.309
	1.467
300 pg/ml	0.755	0.718	0.599
	0.68
150 pg/ml	0.375	0.389	0.27
	0.403
75 pg/ml	0.231	0.246	0.127
	0.261
37.5 pg/ml	0.179	0.18	0.061
	0.181
18.75 pg/ml	0.145	0.146	0.027
	0.146
0 pg/ml	0.114	0.119	0
	0.124

We measured random samples of BPE217 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	188	17.1	9.1
Intra-assay	2	20	384	35.2	9.2
Intra-assay	3	20	853	42.3	5
Inter-assay	1	20	189	18	9.5
Inter-assay	2	20	387	33.1	8.5
Inter-assay	3	20	854	63.3	7.4

> Protocol Booklet

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