Mouse Dickkopf-Related Protein 3, Dkk3 Primacu™ ELISA Kit

Product

OneStep ELISA kit
Primacu ELISA kit
Antibodies
Biochemicals
Recombinant proteins

Service

Custom ELISA assay services
Bulk quote

Research area

Blood coagulation
Metabolism
Cancer
Microbiology
Cardiovascular
Neuroscience
Cell biology
Signal transduction
Developmental biology
Stem cells
Epigenetics and nuclear signaling
Tags & cell markers
Immunology

Resources

Protocols
FAQs
Product brochure
Promotion
Troubleshooting guides
About ELISAs

About us

Company profile
Worldwide distributors
Manufacturing service

Home > Product > ELISA Primacu > Mouse Dickkopf-Related Protein 3, Dkk3 Primacu™ ELISA Kit

Mouse Dickkopf-Related Protein 3, Dkk3 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.86 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 6.86 pg/mL

Detection range: 15.63-1000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Dickkopf-related protein 3

Code

BPE302

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

6.86 pg/mL

Detection range

15.63-1000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.

UniProt accession

Q9QUN9

MASS（Da）

38388

GeneID

50781

Gene names

Dkk3

Research area

Stem cells,Signal transduction

Target protein

DKK3

Components

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

Protocol data

These standard curves of BPE302 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1000 pg/ml	2.791	2.694	2.607
	2.597
500 pg/ml	1.382	1.377	1.29
	1.372
250 pg/ml	0.71	0.757	0.67
	0.804
125 pg/ml	0.431	0.429	0.342
	0.427
62.5 pg/ml	0.256	0.26	0.173
	0.265
31.25 pg/ml	0.183	0.174	0.087
	0.165
15.625 pg/ml	0.125	0.124	0.037
	0.123
0 pg/ml	0.088	0.087	0
	0.086

Precision

We measured random samples of BPE302 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	74.4	6.9	9.3
Intra-assay	2	20	257	25.5	9.9
Intra-assay	3	20	597	57.8	9.7
Inter-assay	1	20	74.9	8.4	11.2
Inter-assay	2	20	254	27.2	10.7
Inter-assay	3	20	592	53.1	9

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

No reviews

Associated products

Datasheet

Size

Code

Price

5*96T

BPE302

$2320.00
96T

BPE302

$580.00
15*96T

BPE302

$6525.00

Add to Cart

Lead time: Within two weeks

Custom quote | Bulk request

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.