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Home > Product > ELISA Primacu > Human Carboxypeptidase A2, CPA2 Primacu™ ELISA Kit

Human Carboxypeptidase A2, CPA2 Primacu™ ELISA Kit

Human Carboxypeptidase A2, CPA2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.27 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.27 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Carboxypeptidase A2

Code

BPE095

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.27 pg/mL

Detection range

12.5-800 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.

UniProt accession

MASS(Da)

47030

GeneID

1358

Gene names

CPA2

Research area

Cell biology

Target protein

Carboxypeptidase A2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE095 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800 pg/ml 2.859 2.792 2.693
2.724
400 pg/ml 1.604 1.624 1.525
1.643
200 pg/ml 0.806 0.856 0.757
0.906
100 pg/ml 0.472 0.486 0.387
0.499
50 pg/ml 0.276 0.289 0.19
0.301
25 pg/ml 0.181 0.191 0.092
0.2
12.5 pg/ml 0.151 0.144 0.045
0.136
0 pg/ml 0.093 0.099 0
0.104

Precision

We measured random samples of BPE095 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 46.7 3.3 7.2
Intra-assay 2 20 234 10.4 4.5
Intra-assay 3 20 615 28.5 4.6
Inter-assay 1 20 46.8 3.6 7.6
Inter-assay 2 20 235 15.2 6.5
Inter-assay 3 20 613 40.2 6.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE095

    $2320.00

  • 96T

    BPE095

    $580.00

  • 15*96T

    BPE095

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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