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Home > Product > ELISA Primacu > Mouse Carboxypeptidase B1 Primacu™ ELISA Kit

Mouse Carboxypeptidase B1 Primacu™ ELISA Kit

Mouse Carboxypeptidase B1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.51 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.51 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Mouse

Product overview

Full product name

Carboxypeptidase B1

Code

BPE312

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.51 pg/mL

Detection range

12.5-800 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Mouse

Storage

2-8°C

Assay time

3.5h

Background

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Research area

Metabolism,Signal transduction

Target protein

Carboxypeptidase B1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE312 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800 pg/ml 2.792 2.813 2.718
2.834
400 pg/ml 1.616 1.569 1.474
1.522
200 pg/ml 0.835 0.847 0.752
0.859
100 pg/ml 0.435 0.467 0.372
0.499
50 pg/ml 0.281 0.295 0.2
0.309
25 pg/ml 0.18 0.187 0.092
0.194
12.5 pg/ml 0.143 0.142 0.047
0.141
0 pg/ml 0.091 0.095 0
0.099

Precision

We measured random samples of BPE312 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 80.4 1.8 2.3
Intra-assay 2 20 167 3.9 2.3
Intra-assay 3 20 419 13.5 3.2
Inter-assay 1 20 80.1 6.6 8.2
Inter-assay 2 20 168 13.6 8.1
Inter-assay 3 20 418 30.3 7.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE312

    $2320.00

  • 96T

    BPE312

    $580.00

  • 15*96T

    BPE312

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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