Full product name

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Assay type

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Sample type

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Assay time

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Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE312 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800 pg/ml	2.792	2.813	2.718
	2.834
400 pg/ml	1.616	1.569	1.474
	1.522
200 pg/ml	0.835	0.847	0.752
	0.859
100 pg/ml	0.435	0.467	0.372
	0.499
50 pg/ml	0.281	0.295	0.2
	0.309
25 pg/ml	0.18	0.187	0.092
	0.194
12.5 pg/ml	0.143	0.142	0.047
	0.141
0 pg/ml	0.091	0.095	0
	0.099

We measured random samples of BPE312 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	80.4	1.8	2.3
Intra-assay	2	20	167	3.9	2.3
Intra-assay	3	20	419	13.5	3.2
Inter-assay	1	20	80.1	6.6	8.2
Inter-assay	2	20	168	13.6	8.1
Inter-assay	3	20	418	30.3	7.3

> Protocol Booklet

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