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Home > Product > ELISA Primacu > Rhesus Low Affinity Immunoglobulin Gamma Fc Region Receptor Primacu™ ELISA Kit

Rhesus Low Affinity Immunoglobulin Gamma Fc Region Receptor Primacu™ ELISA Kit

Rhesus Low Affinity Immunoglobulin Gamma Fc Region Receptor Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.96 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rhesus

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 2.96 pg/mL

Detection range: 12.5-800 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rhesus

Product overview

Full product name

Low affinity immunoglobulin gamma Fc region receptor III

Code

BPE365

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

2.96 pg/mL

Detection range

12.5-800 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rhesus

Storage

2-8°C

Assay time

3.5h

Background

Signal transduction,Immunology bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.

Research area

Immunology,Stem cells

Target protein

CD16

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE365 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800 pg/ml 2.226 2.248 2.137
2.27
400 pg/ml 1.26 1.279 1.168
1.298
200 pg/ml 0.742 0.729 0.618
0.716
100 pg/ml 0.381 0.408 0.297
0.434
50 pg/ml 0.275 0.27 0.159
0.265
25 pg/ml 0.192 0.187 0.076
0.182
12.5 pg/ml 0.153 0.149 0.038
0.145
0 pg/ml 0.108 0.111 0
0.114

Precision

We measured random samples of BPE365 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 37.4 1.3 3.6
Intra-assay 2 20 121 4 3.3
Intra-assay 3 20 603 21.9 3.6
Inter-assay 1 20 37.3 2.1 5.7
Inter-assay 2 20 121 5.6 4.6
Inter-assay 3 20 597 23.4 3.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE365

    $2320.00

  • 96T

    BPE365

    $580.00

  • 15*96T

    BPE365

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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