Full product name

Code

Assay type

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Detection range

Sample type

Species

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Assay time

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Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE365 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800 pg/ml	2.226	2.248	2.137
	2.27
400 pg/ml	1.26	1.279	1.168
	1.298
200 pg/ml	0.742	0.729	0.618
	0.716
100 pg/ml	0.381	0.408	0.297
	0.434
50 pg/ml	0.275	0.27	0.159
	0.265
25 pg/ml	0.192	0.187	0.076
	0.182
12.5 pg/ml	0.153	0.149	0.038
	0.145
0 pg/ml	0.108	0.111	0
	0.114

We measured random samples of BPE365 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	37.4	1.3	3.6
Intra-assay	2	20	121	4	3.3
Intra-assay	3	20	603	21.9	3.6
Inter-assay	1	20	37.3	2.1	5.7
Inter-assay	2	20	121	5.6	4.6
Inter-assay	3	20	597	23.4	3.9

> Protocol Booklet

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