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Home > Product > ELISA Primacu > Human Prolactin Receptor, PRLR Primacu™ ELISA Kit

Human Prolactin Receptor, PRLR Primacu™ ELISA Kit

Human Prolactin Receptor, PRLR Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.64 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.64 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Prolactin receptor

Code

BPE057

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.64 pg/mL

Detection range

7.81-500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the type â… cytokine receptor superfamily, and contains two fibronectin type-â…¢ domains. All class 1 ligands activate their respective receptors by clustering mechanisms. Ligand binding results in the transmembrane PRLR dimerization, followed by phosphorylation and activation of the molecules involved in the signaling pathways, such as Jak-STAT, Ras/Raf/MAPK. The PRLR contains no intrinsic tyrosine kinase cytoplasmic domain but associates with a cytoplasmic tyrosine kinase, JAK2. PRLR mainly serves as the receptor for the pituitary hormone prolactin (PRL), a secreted hormone that affects reproduction and homeostasis in vertebrates. PRLR can be regulated by an interplay of two different mechanisms, PRL or ovarian steroid hormones independently or in combination in a tissue-specific manner. The role of the hormone prolactin (PRL) in the pathogenesis of breast cancer is mediated by its cognate receptor (PRLR). Ubiquitin-dependent degradation of the PRLR that negatively regulates PRL signaling is triggered by PRL-mediated phosphorylation of PRLR on Ser349 followed by the recruitment of the beta-transducin repeats-containing protein (beta-TrCP) ubiquitin-protein isopeptide ligase. which altered PRLR stability may directly influence the pathogenesis of breast cancer.

UniProt accession

MASS(Da)

69506

GeneID

5618

Gene names

PRLR

Research area

Signal transduction

Target protein

Prolactin Receptor

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE057 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
500 pg/ml 1.813 1.806 1.719
1.799
250 pg/ml 0.987 0.969 0.882
0.951
125 pg/ml 0.506 0.516 0.429
0.526
62.5 pg/ml 0.286 0.276 0.189
0.266
31.25 pg/ml 0.179 0.184 0.097
0.189
15.625 pg/ml 0.123 0.124 0.037
0.125
7.8125 pg/ml 0.107 0.106 0.019
0.105
0 pg/ml 0.085 0.087 0
0.089

Precision

We measured random samples of BPE057 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 46.9 1.7 3.6
Intra-assay 2 20 95.7 2.9 3.1
Intra-assay 3 20 330 10.1 3.1
Inter-assay 1 20 47.4 3.9 8.2
Inter-assay 2 20 95.3 7.6 8
Inter-assay 3 20 331 15.8 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE057

    $2320.00

  • 96T

    BPE057

    $580.00

  • 15*96T

    BPE057

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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