Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE113 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500 pg/ml	2.012	2.077	1.947
	2.142
250 pg/ml	1.096	1.084	0.954
	1.072
125 pg/ml	0.595	0.597	0.467
	0.599
62.5 pg/ml	0.356	0.378	0.248
	0.401
31.25 pg/ml	0.249	0.254	0.124
	0.259
15.625 pg/ml	0.178	0.188	0.058
	0.199
7.8125 pg/ml	0.155	0.16	0.03
	0.165
0 pg/ml	0.136	0.13	0
	0.124

We measured random samples of BPE113 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	62.5	1.6	2.6
Intra-assay	2	20	145	4.8	3.3
Intra-assay	3	20	346	11.6	3.4
Inter-assay	1	20	63.1	5.2	8.3
Inter-assay	2	20	144	9.8	6.8
Inter-assay	3	20	348	14.9	4.3

> Protocol Booklet

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