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Home > Product > ELISA Primacu > Human Brorin, VWC2 Primacu™ ELISA Kit

Human Brorin, VWC2 Primacu™ ELISA Kit

Human Brorin, VWC2 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.52 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.52 pg/mL

Detection range: 7.81-500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Brorin

Code

BPE241

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.52 pg/mL

Detection range

7.81-500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Brorin, also known as brain-specific chordin-like protein, von Willebrand factor C domain-containing protein 2 and VWC2, is a secreted protein that contains two VWFC domains. VWC2 / Brorin is a BMP antagonist that may play a role in neural development. It promotes cell adhesion. VWC2 / Brorin is a unique member of the chordin family. It inhibited the activity of bone morphogenetic protein 2 (BMP2) and BMP6 in mouse preosteoblastic MC3T3-E1 cells. Mouse Brorin was predominantly expressed in neural tissues in embryos and also predominantly expressed in the adult brain. In the brain, the expression was detected in neurons, but not glial cells. The neural tissue-specific expression profile of Brorin is quite distinct from that of any other member of the Chordin family. VWC2 / Brorin protein promoted neurogenesis, but not astrogenesis, in mouse neural precursor cells. VWC2 / Brorin is a novel secreted BMP antagonist that potentially plays roles in neural development and functions.

UniProt accession

MASS(Da)

35282

GeneID

375567

Gene names

VWC2

Research area

metabolism

Target protein

VWC2

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE241 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
500 pg/ml 1.669 1.662 1.564
1.656
250 pg/ml 1.073 1.117 1.019
1.161
125 pg/ml 0.734 0.719 0.621
0.704
62.5 pg/ml 0.423 0.421 0.323
0.419
31.25 pg/ml 0.276 0.261 0.163
0.246
15.625 pg/ml 0.167 0.169 0.071
0.172
7.8125 pg/ml 0.126 0.135 0.037
0.144
0 pg/ml 0.092 0.098 0
0.105

Precision

We measured random samples of BPE241 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 36.6 1.7 4.7
Intra-assay 2 20 96.4 3.4 3.5
Intra-assay 3 20 301 6.4 2.1
Inter-assay 1 20 36.5 1.5 4.2
Inter-assay 2 20 96.6 2.8 2.8
Inter-assay 3 20 301 7 2.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE241

    $2320.00

  • 96T

    BPE241

    $580.00

  • 15*96T

    BPE241

    $6525.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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