Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE293 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
500 pg/ml	1.647	1.656	1.521
	1.664
250 pg/ml	1.067	1.088	0.953
	1.108
125 pg/ml	0.679	0.707	0.572
	0.734
62.5 pg/ml	0.448	0.441	0.306
	0.433
31.25 pg/ml	0.308	0.298	0.163
	0.287
15.625 pg/ml	0.209	0.215	0.08
	0.221
7.8125 pg/ml	0.168	0.173	0.038
	0.177
0 pg/ml	0.131	0.135	0
	0.139

We measured random samples of BPE293 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	43.1	2.5	5.9
Intra-assay	2	20	123	4.2	3.4
Intra-assay	3	20	373	20.5	5.5
Inter-assay	1	20	43.3	2.8	6.4
Inter-assay	2	20	124	12.6	10.2
Inter-assay	3	20	376	12.4	3.3

> Protocol Booklet

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