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Home > Product > ELISA Primacu > Human Azurocidin, AZU1 Primacu™ ELISA Kit

Human Azurocidin, AZU1 Primacu™ ELISA Kit

Human Azurocidin, AZU1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.4 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 1.4 pg/mL

Detection range: 6.25-400 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Azurocidin

Code

BPE116

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

1.4 pg/mL

Detection range

6.25-400 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Azurocidin (AZU1), also known as heparin-binding protein (HBP) or cationic antimicrobial protein 37 (CAP37), is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. It thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.

UniProt accession

MASS(Da)

26886

GeneID

566

Gene names

AZU1

Research area

Cardiovascular,Immunology,Microbiology

Target protein

Azurocidin

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE116 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400 pg/ml 2.068 2.145 2.055
2.223
200 pg/ml 1.146 1.206 1.116
1.266
100 pg/ml 0.639 0.67 0.58
0.702
50 pg/ml 0.412 0.394 0.304
0.376
25 pg/ml 0.235 0.244 0.154
0.254
12.5 pg/ml 0.173 0.167 0.077
0.162
6.25 pg/ml 0.13 0.131 0.041
0.133
0 pg/ml 0.091 0.09 0
0.09

Precision

We measured random samples of BPE116 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 17.5 0.6 3.4
Intra-assay 2 20 83.2 2.3 2.8
Intra-assay 3 20 277 9.2 3.3
Inter-assay 1 20 17.7 1.6 9
Inter-assay 2 20 84.1 6.9 8.2
Inter-assay 3 20 278 24.5 8.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE116

    $2320.00

  • 96T

    BPE116

    $580.00

  • 15*96T

    BPE116

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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