Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE116 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
400 pg/ml	2.068	2.145	2.055
	2.223
200 pg/ml	1.146	1.206	1.116
	1.266
100 pg/ml	0.639	0.67	0.58
	0.702
50 pg/ml	0.412	0.394	0.304
	0.376
25 pg/ml	0.235	0.244	0.154
	0.254
12.5 pg/ml	0.173	0.167	0.077
	0.162
6.25 pg/ml	0.13	0.131	0.041
	0.133
0 pg/ml	0.091	0.09	0
	0.09

We measured random samples of BPE116 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	17.5	0.6	3.4
Intra-assay	2	20	83.2	2.3	2.8
Intra-assay	3	20	277	9.2	3.3
Inter-assay	1	20	17.7	1.6	9
Inter-assay	2	20	84.1	6.9	8.2
Inter-assay	3	20	278	24.5	8.8

> Protocol Booklet

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