Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE342 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
200 pg/ml	2.982	3.085	2.969
	3.188
100 pg/ml	1.609	1.55	1.434
	1.491
50 pg/ml	0.721	0.734	0.618
	0.747
25 pg/ml	0.403	0.39	0.274
	0.377
12.5 pg/ml	0.24	0.252	0.136
	0.264
6.25 pg/ml	0.173	0.167	0.051
	0.161
3.125 pg/ml	0.136	0.14	0.024
	0.144
0 pg/ml	0.108	0.116	0
	0.124

We measured random samples of BPE342 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	12.3	0.5	4
Intra-assay	2	20	33.1	0.82	2.5
Intra-assay	3	20	107	3.4	3.1
Inter-assay	1	20	12.3	1	8.2
Inter-assay	2	20	33.2	2.7	8.3
Inter-assay	3	20	107	6.8	6.4

> Protocol Booklet

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