Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE159 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
150 pg/ml	2.592	2.644	2.554
	2.696
75 pg/ml	1.45	1.43	1.34
	1.41
37.5 pg/ml	0.798	0.773	0.683
	0.748
18.75 pg/ml	0.392	0.414	0.324
	0.436
9.375 pg/ml	0.244	0.246	0.156
	0.248
4.6875 pg/ml	0.158	0.162	0.072
	0.167
2.34375 pg/ml	0.122	0.123	0.033
	0.125
0 pg/ml	0.095	0.09	0
	0.086

We measured random samples of BPE159 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	23.9	0.69	2.9
Intra-assay	2	20	50.8	1.3	2.7
Intra-assay	3	20	106	3.4	3.2
Inter-assay	1	20	23.9	1.7	7.1
Inter-assay	2	20	51.1	3.4	6.7
Inter-assay	3	20	105	6.7	6.4

> Protocol Booklet

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