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Home > Product > ELISA Primacu > Human Interleukin-3, IL3 Primacu™ ELISA Kit

Human Interleukin-3, IL3 Primacu™ ELISA Kit

Human Interleukin-3, IL3 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.5 pg/mL

Detection range: 2.34-150 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 0.5 pg/mL

Detection range: 2.34-150 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Interleukin-3

Code

BPE231

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

0.5 pg/mL

Detection range

2.34-150 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

IL3 (interleukin 3), also known as IL-3, is a potent growth-promoting cytokine that belongs to the IL-3 family. IL3/IL-3 also belongs to the group of interleukins. Interleukins are produced by a wide variety of body cells. The function of the immune system depends in a large part on interleukins, and rare deficiencies of a number of them have been described, all featuring autoimmune diseases or immune deficiency. The majority of interleukins are synthesized by helper CD4+ T lymphocytes, as well as through monocytes, macrophages, and endothelial cells. They promote the development and differentiation of T, B, and hematopoietic cells. IL3/IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation, and apoptosis. IL3/IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.

UniProt accession

MASS(Da)

17233

GeneID

3562

Gene names

IL3

Research area

Immunology,Neuroscience,Stem cells,Cancer

Target protein

IL3

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE231 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
150 pg/ml 2.39 2.476 2.389
2.562
75 pg/ml 1.335 1.393 1.306
1.451
37.5 pg/ml 0.739 0.746 0.659
0.753
18.75 pg/ml 0.427 0.423 0.336
0.419
9.375 pg/ml 0.258 0.252 0.165
0.245
4.6875 pg/ml 0.163 0.172 0.085
0.18
2.34375 pg/ml 0.126 0.133 0.046
0.14
0 pg/ml 0.083 0.087 0
0.091

Precision

We measured random samples of BPE231 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 10.2 0.29 2.9
Intra-assay 2 20 32.9 1.2 3.6
Intra-assay 3 20 84.4 2.2 2.6
Inter-assay 1 20 10.3 0.39 3.8
Inter-assay 2 20 33.3 2.5 7.5
Inter-assay 3 20 84.2 3.2 3.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE231

    $2040.00

  • 96T

    BPE231

    $480.00

  • 15*96T

    BPE231

    $5400.00

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Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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