Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE231 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
150 pg/ml	2.39	2.476	2.389
	2.562
75 pg/ml	1.335	1.393	1.306
	1.451
37.5 pg/ml	0.739	0.746	0.659
	0.753
18.75 pg/ml	0.427	0.423	0.336
	0.419
9.375 pg/ml	0.258	0.252	0.165
	0.245
4.6875 pg/ml	0.163	0.172	0.085
	0.18
2.34375 pg/ml	0.126	0.133	0.046
	0.14
0 pg/ml	0.083	0.087	0
	0.091

We measured random samples of BPE231 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	10.2	0.29	2.9
Intra-assay	2	20	32.9	1.2	3.6
Intra-assay	3	20	84.4	2.2	2.6
Inter-assay	1	20	10.3	0.39	3.8
Inter-assay	2	20	33.3	2.5	7.5
Inter-assay	3	20	84.2	3.2	3.7

> Protocol Booklet

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