Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS(Da)
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E5995Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 1600ng/L | 1.667 | 1.689 | 1.652 |
| 1.711 | |||
| 800ng/L | 0.936 | 0.912 | 0.875 |
| 0.888 | |||
| 400ng/L | 0.532 | 0.561 | 0.524 |
| 0.591 | |||
| 200ng/L | 0.268 | 0.237 | 0.2 |
| 0.206 | |||
| 100ng/L | 0.149 | 0.129 | 0.092 |
| 0.11 | |||
| 0ng/L | 0.032 | 0.037 | 0 |
| 0.042 |
Precision
We measured random samples of E5995Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 1360.27 | 42.17 | 3.1 |
| Intra-Assay | 2 | 18 | 905.26 | 46.16 | 5.1 |
| Intra-Assay | 3 | 18 | 481.11 | 26.73 | 5.56 |
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