Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
20000 pg/ml | 2.418 | 2.463 | 2.381 |
2.507 | |||
10000 pg/ml | 1.3 | 1.316 | 1.234 |
1.331 | |||
5000 pg/ml | 0.743 | 0.706 | 0.624 |
0.668 | |||
2500 pg/ml | 0.346 | 0.371 | 0.289 |
0.395 | |||
1250 pg/ml | 0.21 | 0.224 | 0.142 |
0.237 | |||
625 pg/ml | 0.144 | 0.147 | 0.065 |
0.15 | |||
312.5 pg/ml | 0.103 | 0.108 | 0.026 |
0.112 | |||
0 pg/ml | 0.084 | 0.082 | 0 |
0.079 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 1884 | 146 | 7.7 |
Intra-assay | 2 | 20 | 4095 | 223 | 5.4 |
Intra-assay | 3 | 20 | 11374 | 459 | 4 |
Inter-assay | 1 | 20 | 1865 | 199 | 10.7 |
Inter-assay | 2 | 20 | 4108 | 346 | 8.4 |
Inter-assay | 3 | 20 | 11391 | 798 | 7 |
Protocols
References
Customer reviews and Q&As