Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
5000 pg/ml | 2.268 | 2.384 | 2.303 |
2.5 | |||
2500 pg/ml | 1.293 | 1.231 | 1.15 |
1.169 | |||
1250 pg/ml | 0.643 | 0.625 | 0.544 |
0.607 | |||
625 pg/ml | 0.392 | 0.373 | 0.292 |
0.354 | |||
312.5 pg/ml | 0.215 | 0.228 | 0.147 |
0.24 | |||
156.25 pg/ml | 0.141 | 0.151 | 0.07 |
0.161 | |||
78.125 pg/ml | 0.104 | 0.101 | 0.02 |
0.097 | |||
0 pg/ml | 0.08 | 0.081 | 0 |
0.081 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 494 | 33.1 | 6.7 |
Intra-assay | 2 | 20 | 1301 | 67.8 | 5.2 |
Intra-assay | 3 | 20 | 2890 | 160 | 5.6 |
Inter-assay | 1 | 20 | 491 | 37.9 | 7.7 |
Inter-assay | 2 | 20 | 1310 | 93.3 | 7.1 |
Inter-assay | 3 | 20 | 2872 | 152 | 5.3 |
Protocols
References
Customer reviews and Q&As