Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
2000 pg/ml | 2.536 | 2.482 | 2.386 |
2.427 | |||
1000 pg/ml | 1.398 | 1.392 | 1.296 |
1.385 | |||
500 pg/ml | 0.806 | 0.778 | 0.682 |
0.749 | |||
250 pg/ml | 0.428 | 0.447 | 0.351 |
0.465 | |||
125 pg/ml | 0.279 | 0.271 | 0.175 |
0.262 | |||
62.5 pg/ml | 0.185 | 0.189 | 0.093 |
0.192 | |||
31.25 pg/ml | 0.145 | 0.156 | 0.06 |
0.166 | |||
0 pg/ml | 0.097 | 0.096 | 0 |
0.094 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 69.9 | 2.5 | 3.6 |
Intra-assay | 2 | 20 | 333 | 10.9 | 3.3 |
Intra-assay | 3 | 20 | 1331 | 72.2 | 5.4 |
Inter-assay | 1 | 20 | 69.1 | 5.7 | 8.2 |
Inter-assay | 2 | 20 | 335 | 20.7 | 6.2 |
Inter-assay | 3 | 20 | 1345 | 94.1 | 7 |
Protocols
Product publications
Customer reviews and Q&As