Product overview
Components
Components | Quantity |
---|---|
Pre-coated ELISA Plate | 12 × 8 well strips × 1 |
Standard Solution | 0.2mL × 1 vail |
Dilution Buffer | 20mL × 1 vail |
Stop Solution | 6mL × 1 vail |
Substrate Solution A | 12mL × 1 vail |
Substrate Solution B | 12mL × 1 vail |
Wash Buffer Concentrate (25x) | 20mL × 1 vail |
Detection Antibody Concentrate | 0.6mL × 1 vail |
Protocol data
These standard curves of are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Concentration | O.D. | Average | Corrected |
1000 pg/ml | 2.419 | 2.427 | 2.329 |
2.436 | |||
500 pg/ml | 1.283 | 1.33 | 1.232 |
1.377 | |||
250 pg/ml | 0.763 | 0.732 | 0.634 |
0.701 | |||
125 pg/ml | 0.392 | 0.409 | 0.311 |
0.426 | |||
62.5 pg/ml | 0.242 | 0.236 | 0.138 |
0.231 | |||
31.25 pg/ml | 0.174 | 0.166 | 0.068 |
0.159 | |||
15.625 pg/ml | 0.119 | 0.124 | 0.026 |
0.129 | |||
0 pg/ml | 0.093 | 0.098 | 0 |
0.104 |
Precision
We measured random samples of within the same batch/lot to ensure the consistency of the kits' performances.
Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
Intra-assay | 1 | 20 | 44 | 1.2 | 2.8 |
Intra-assay | 2 | 20 | 176 | 3.4 | 2 |
Intra-assay | 3 | 20 | 678 | 22.6 | 3.3 |
Inter-assay | 1 | 20 | 44.4 | 3.2 | 7.2 |
Inter-assay | 2 | 20 | 177 | 11.9 | 6.7 |
Inter-assay | 3 | 20 | 680 | 45.9 | 6.8 |
Protocols
References
Customer reviews and Q&As