Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
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Storage
Assay time
Background
Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress (By similarity).
UniProt accession
MASS（Da）
GeneID
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Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E2104Mo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8000μU/mL | 2.115 | 2.067 | 2.021 |
| 2.02 | |||
| 4000μU/mL | 1.521 | 1.442 | 1.396 |
| 1.364 | |||
| 2000μU/mL | 0.56 | 0.67 | 0.623 |
| 0.78 | |||
| 1000μU/mL | 0.371 | 0.411 | 0.364 |
| 0.452 | |||
| 500μU/mL | 0.147 | 0.151 | 0.104 |
| 0.155 | |||
| 0μU/mL | 0.038 | 0.047 | 0 |
| 0.055 |
Precision
We measured random samples of E2104Mo within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 3181.1 | 171.34 | 5.4 |
| Intra-Assay | 2 | 18 | 3818.7 | 129.88 | 3.4 |
| Intra-Assay | 3 | 18 | 1307.2 | 56.59 | 4.3 |
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