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Home > Product > ELISA Kit > Rat Acetyl-CoA Carboxylase 1, ACC1 ELISA Kit

Rat Acetyl-CoA Carboxylase 1, ACC1 ELISA Kit

Rat Acetyl-CoA Carboxylase 1, ACC1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.03ng/ml

Detection range: 0.05-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Acetyl-Coa Carboxylase 1

Code

E0970Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.03ng/ml

Detection range

0.05-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This is a 2 steps reaction starting with the ATP-dependent carboxylation of the biotin carried by the biotin carboxyl carrier (BCC) domain followed by the transfer of the carboxyl group from carboxylated biotin to acetyl-CoA.

UniProt accession

MASS(Da)

265,194

GeneID

60581

Synonyms

ACACA;ACC1;ACC-alpha;Acetyl-CoA carboxylase 1

Gene names

ACACA

Research area

Others

Target protein

ACACA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Acetyl-CoA Carboxylase 1, ACC1 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.214 2.266 2.208
2.318
12ng/ml 1.408 1.482 1.424
1.555
6ng/ml 0.865 0.819 0.761
0.774
3ng/ml 0.431 0.438 0.38
0.445
1.5ng/ml 0.287 0.258 0.2
0.229
0ng/ml 0.046 0.058 0
0.07

Precision

We measured random samples of E0970Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 4.5 0.26 5.8
Intra-Assay 2 18 12 0.3 2.5
Intra-Assay 3 18 9.7 0.55 5.7

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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