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Home > Product > ELISA Kit > Human Liver Carboxylesterase 1, CES1 ELISA Kit

Human Liver Carboxylesterase 1, CES1 ELISA Kit

Human Liver Carboxylesterase 1, CES1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.46ng/ml

Detection range: 1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.46ng/ml

Detection range: 1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Liver Carboxylesterase 1

Code

E2395Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.46ng/ml

Detection range

1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs (PubMed: 7980644, PubMed: 9169443, PubMed: 9490062, PubMed: 18762277). Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester (PubMed: 7980644, PubMed: 9169443, PubMed: 9490062, PubMed: 18762277). Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine (PubMed: 7980644). Catalyzes the transesterification of cocaine to form cocaethylene (PubMed: 7980644). Displays fatty acid ethyl ester Synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate (PubMed: 7980644). Converts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes of 2-arachidonoylglycerol and prostaglandins (PubMed: 21049984). Hydrolyzes cellular cholesteryl esters to free cholesterols and promotes reverse cholesterol transport (RCT) by facilitating both the initial and final steps in the process (PubMed: 18762277, PubMed: 16024911, PubMed: 11015575, PubMed: 16971496). First of all, allows free cholesterol efflux from macrophages to extracellular cholesterol acceptors and secondly, releases free cholesterol from lipoprotein-delivered cholesteryl esters in the liver for bile acid synthesis or direct secretion into the bile (PubMed: 18762277, PubMed: 18599737, PubMed: 16971496).

UniProt accession

MASS(Da)

62,521

GeneID

1066

Synonyms

ACAT;Acyl-coenzyme A:cholesterol acyltransferase;Brain carboxylesterase hBr1;Carboxylesterase 1;CE-1;CEH;CES 1;CES1;Cholesteryl ester hydrolase;Cocaine carboxylesterase;Egasyn;hCE-1;HMSE;Liver carboxylesterase 1;Methylumbelliferyl-acetate deacetylase 1;Monocyte/macrophage serine esterase;REH;Retinyl ester hydrolase;Serine esterase 1;TGH;Triacylglycerol hydrolase

Gene names

CES1

Research area

Cardiovascular

Target protein

CES1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2395Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.305 2.172 2.122
2.04
12ng/ml 1.486 1.397 1.347
1.308
6ng/ml 0.845 0.855 0.805
0.866
3ng/ml 0.421 0.485 0.435
0.548
1.5ng/ml 0.299 0.302 0.252
0.304
0ng/ml 0.061 0.05 0
0.039

Precision

We measured random samples of E2395Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 15.2 0.64 4.2
Intra-Assay 2 18 8.3 0.43 5.1
Intra-Assay 3 18 4.1 0.24 5.8

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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